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| Registros recuperados: 425 | |
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| Gkaravela,Lamprini; Papadimitriou-Olivgeris,Matthaios; Foka,Antigoni; Kolonitsiou,Fevronia; Spiliopoulou,Anastasia; Charokopos,Nikolaos; Voulgaridis,Apostolos; Tsiamita,Maria; Marangos,Markos; Anastassiou,Evangelos D.; Spiliopoulou,Iris. |
| ABSTRACT Early diagnosis of tuberculosis is of major clinical importance. Among 4733 clinical specimens collected from 3363 patients and subjected to Ziehl-Neelsen microscopy, 4109 were inoculated onto Löwenstein-Jensen slants and 3139 in Bactec/9000MB. Polymerase Chain Reaction (PCR) was performed in 3139 specimens, whereas, a genotypic assay was directly applied in 93 Mycobacterium tuberculosis complex PCR-positive for isoniazid and rifampicin resistance detection specimens (GenoType MTBDRplus). Recovered M. tuberculosis isolates (64) as well as, 21 more sent from Regional Hospitals were tested for antimycobacterial resistance with a phenotypic (manual MGIT-SIRE) and a genotypic assay (GenoType MTBDRplus). PCR in the clinical specimens showed excellent... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mycobacterium tuberculosis; PCR; GenoType MTBDRplus. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000400785 |
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| Barros,M.R.; Andreatti Filho,R.L.; Oliveira,D.E.; Lima,E.T.; Crocci,A.J.. |
| Lactobacilos foram isolados do inglúvio e cecos de reprodutoras pesadas e caracterizados como Gram-positivo, catalase negativo, produtores de gás em glicose e não produtores de H2S em triple sugar iron e pela fermentação de carboidratos. Utilizaram-se os iniciadores: Lac 1/23-10C para detecção de Lactobacillus acidophilus, L. crispatus, L. amylovorus, L. gasseri, L. helveticus e L. jensenii; Lac 2/LU-1' para L. acidophilus; Fer 3/Fer 4 para L. fermentum; Reu 1/Reu 2 para L. reuteri e Sal 1 e Sal 2 para L. salivarius. L. reuteri e L. salivarius foram identificados pela reação em cadeia de polimerase (PCR) e pelo teste bioquímico, enquanto L. acidophilus, L. fermentum e Lactobacillus sp. somente pelo teste bioquímico. Os resultados obtidos na PCR foram mais... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Galinha; Reprodutora de frango de corte; Identificação bioquímica; Lactobacillus; PCR. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352009000200006 |
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| Singh,Harkanwaldeep; Rathore,R.S; Singh,Satparkash; Cheema,Pawanjit Singh. |
| In the present study, the efficacy of polymerase chain reaction (PCR) based on mapA gene of C. jejuni was tested for detection of Campylobacter jejuni in naturally infected as well as spiked faecal and food samples of human and animal origin. Simultaneously, all the samples were subjected to the cultural isolation of organism and biochemical characterization. The positive samples resulted in the amplification of a DNA fragment of size ~589 bp in PCR assay whereas the absence of such amplicon in DNA extracted from E. coli, Listeria, Salmonella and Staphylococcus confirmed the specificity of the primers. Of randomly collected 143 faecal samples comprising human diarrheic stools (43), cattle diarrheic faeces (48) and poultry faecal swabs (52) only 4, 3 and 8,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Campylobacter jejuni; Isolation; PCR; Spiking. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100022 |
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| Aksoy,A; El Kahlout,KEM; Yardimci,H. |
| ABSTRACT Using disinfectants in poultry houses is a common practice to ban the zoonotic pathogens like Salmonella. A major concern in using disinfectants is the emergence of bacteria strains that resist some disinfectants. This phenomenon is manifested in the resistance of some Salmonella serotypes against quaternary ammonium compounds. Such resistance is attributed to qacEΔ1 gene which may be possessed by some Salmonella serotypes. This work aimed to evaluate the resistance of Salmonella serotypes (S. Typhimurium, S. Infantis and S. Entiridis) against different disinfectants (benzalkonium chloride, iodine, gluteraldehyde and hydrogen peroxide). The effect of the disinfectants were evaluated by treatment of the bacteria with different concentrations... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Disinfectants; PCR; Poultry; Resistance; Salmonella. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2020000100301 |
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| Campos,Karoline Rodrigues; Gonçalves,Maria Gisele; Costa,Nadia Aparecida; Caterino-de-Araujo,Adele. |
| ABSTRACT The present study evaluated several techniques currently available (commercial kits and in-house assays) for diagnosing human T lymphotropic viruses types 1 and 2 in two groups of patients enrolled at HIV/AIDS specialized care services in São Paulo: Group 1 (G1), n = 1608, 1237 male/371 female, median age 44.3 years old, majority using highly active antiretroviral therapy (HAART); G2, n = 1383, 930 male/453 female, median age of 35.6 years old, majority HAART naïve. Enzyme immunoassays [(EIA) Murex and Gold ELISA] were employed for human T lymphotropic viruses types 1 and 2 screening; Western blotting (WB), INNO-LIA (LIA), real-time PCR pol (qPCR), and nested-PCR-RFLP (tax) were used to confirm infection. Samples were considered human T... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: HTLV-1/2; HIV-1; Coinfection; Diagnosis; Immunoblotting; PCR; HAART. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000300297 |
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| Resende,M.V.; Lucio,A.C.; Perini,A.P.; Oliveira,L.Z.; Almeida,A.O.; Alves,B.C.A.; Moreira-Filho,C.A.; Santos,I.W.; Hossepian de Lima,V.F.M.. |
| The objective of the present study was to determine the sperm enrichment with X-bearing spermatozoa, after one centrifugation in a Percoll or OptiPrep continuous density gradient, using quantitative real-time polymerase chain reaction (qPCR) of sperm DNA and resultant in vitro-produced bovine embryos by PCR. Frozen/thawed sperm was layered on density gradients and the tubes were centrifuged. Supernatants were gently aspirated and the sperm recovered from the bottom of the tubes. Cleavage and blastocyst rates were determined through in vitro production of embryos and PCR was performed to identify the embryos' genetic sex. A difference in blastocyst rate was found in the Percoll treatment compared to OptiPrep (P<0.05). The percentage of female embryos in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine; Sperm sexing; Centrifugation; Embryo sexing; QPCR; PCR. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000300002 |
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| Başpınar,Emel Ödemiş; Dayan,Saim; Bekçibaşı,Muhammed; Tekin,Recep; Ayaz,Celal; Deveci,Özcan; Hoşoğlu,Salih. |
| Abstract Our aim in this study is to compare the standard culture method with the multiplex PCR and the Speed-Oligo® Bacterial Meningitis Test (SO-BMT) – a hybridization-based molecular test method – during the CSF examination of the patients with the pre-diagnosis of acute bacterial meningitis. For the purposes of this study, patients with acute bacterial meningitis treated at the Dicle University Medical Faculty Hospital, Infectious Diseases and Clinical Microbiology Clinic between December 2009 and April 2012 were retrospectively evaluated. The diagnosis of bacterial meningitis was made based on the clinical findings, laboratory test anomalies, CSF analysis results, and the radiological images. Growth was observed in the CSF cultures of 10 out of the 57... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacterial meningitis; Culture; Speed-Oligo; PCR. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000200232 |
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| César,Ana Cristina Gobbo; Cury,Patrícia Maluf; Payão,Spencer Luiz Marques; Liberatore,Paula Rahal; Silva,Ana Elizabete. |
| Helicobacter pylori colonization is associated with chronic gastritis, peptic ulcers, intestinal metaplasia, adenocarcinoma and lymphoma of the stomach. The objective of this study was to compare the results of the routinely used histology with molecular diagnosis for the detection of H. pylori. Eighty samples from gastric lesions (chronic gastritis, atrophic gastritis, gastric ulcer, and intestinal metaplasia), 18 gastric adenocarcinoma and 10 normal mucosa H. pylori-negative (control) samples were obtained. All samples were examined histologically (hematoxylin-eosin and Giemsa staining), and PCR amplifications of the species-specific antigen gene (H3H4) and urease A gene segment (H5H6) of H. pylori were made, using the human gene CYP1A1 for DNA quality... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Helicobacter pylori; Gastric lesions; Adenocarcinoma; Histological diagnosis; PCR. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000100003 |
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| Michelim,Lessandra; Muller,Gabriela; Zacaria,Jucimar; Delamare,Ana Paula Longaray; Costa,Sérgio Olavo Pinto da; Echeverrigaray,Sergio. |
| Proteus mirabilis is one of the most important pathogens associated with complicated urinary tract infections (acute pyelonephritis, bladder infections, kidney stones) and bacteremia, affecting patients with anatomical abnormalities, immunodeficiency, and long-term urinary catheterization. For epidemiological purposes, various molecular typing methods, such as pulse-field gel electrophoresis (PFGE) or ribotyping, have been developed for this pathogen. However, these methods are labor intensive and time-consuming. We evaluated the discriminatory power of several PCR-based fingerprinting methods (RAPD, ISSR, ERIC-PCR, BOX-PCR and rep-PCR) for P. mirabilis clinical isolates. Typing patterns and clustering analysis indicated that RAPD, BOX-PCR and ERIC-PCR... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Proteus mirabilis; Molecular markers; Fingerprinting; PCR. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000500014 |
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| Silva,M.M.O.; Castro,T.X.; Costa,E.M.; Trancoso,T.A.L.; Mendes-de-Almeida,F.; Labarthe,N.V.; Cubel Garcia,R.C.N.. |
| The aim of this study was to evaluate the rapid tests currently used for canine parvovirus (CPV) diagnosis: hemagglutination test (HA), enzyme immunoassay (EIA) and polymerase chain reaction (PCR). A total of 112 fecal samples collected from diarrheic puppies up to one year of age were tested. The EIA was able to detect CPV antigen in 44 samples. By HA, 32 samples tested highly positive with titers >128, eight tested weakly positive (titers 32 and 64) and 72 were negative (titers <16). Using PCR, 57 samples were found positive including 13 EIA-negative and 19 HA-negative samples. The best correlation was observed between EIA and PCR (88.4%). These tests were able to detect all types of CPV, including CPV-2c. Considering that 23%-33% of dogs... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Canine parvovirus; Enteritis; HA; EIA; PCR. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000100023 |
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| Giacomazzi, Sophie; Leroi, Francoise; Joffraud, Jean-jacques. |
| Aims: To compare three bacterial DNA extraction procedures on cold-smoked salmon (CSS) and assess the impact on their efficiency of two physical treatments of the food matrix, ionizing irradiation and freezing. Methods and Results: As molecular methods for bacterial detection have become an important analytical tool, we compared bacterial DNA extraction procedures on CSS. Working with frozen and irradiated CSS, we obtained negative responses from samples known to be highly contaminated. Thus, we decided to study the impact of these two physical treatments on bacterial DNA extraction procedures. The efficiency of bacterial DNA extraction directly from the fish matrix suspension was measured by an rpoB PCR-based reaction. The results demonstrated that the... |
| Tipo: Text |
Palavras-chave: RpoB gene; PCR; Ionizing irradiation; Freezing; DNA extraction; Cold smoked salmon. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-1106.pdf |
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| Lins,Severina Rodrigues de Oliveira; Abreu,Mário Sobral de; Alves,Eduardo; Barbosa,Juliana Franco; Souza,Ricardo Magela de. |
| A mancha manteigosa tem afetado um grande número de plantas de cafeeiro em condições de campo. Sua causa tem sido atribuída a Colletotrichum gloeosporioides, entretanto a sintomatologia da doença na folha não tem sido reproduzida. Neste estudo, relata-se pela primeira vez a associação de Xylella fastidiosa, agente da atrofia dos ramos de cafeeiro, com pecíolo de folhas e hipocótilos obtidos a partir de sementes de plantas com sintomas da mancha manteigosa, através de estudos ultra-estruturais em Microscópio Eletrônico de Varredura (MEV), bem como por PCR (Polymerase Chain Reaction). Para o estudo foram realizados três ensaios. No primeiro, coletaram-se folhas com sintomas de mancha manteigosa e assintomáticas em duas localidades as quais foram preparadas... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mancha manteigosa; Xylella fastidiosa; Microscopia eletrônica de varredura; PCR. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542008000100006 |
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| Soares,Celina Mara; Valadares,Geórgio Freesz; Azeredo,Raquel Monteiro Cordeiro de; Kuaye,Arnaldo Yoshiteru. |
| A avaliação da contaminação ambiental por Bacillus cereus foi realizada em 90 amostras de ar ambiente e em 96 amostras de superfícies de bancadas e de equipamentos, de dois restaurantes institucionais. O microrganismo foi detectado em 84,4% e 44,8% das amostras de ar ambiente e de superfícies, respectivamente. O potencial enterotoxigênico dos isolados foi investigado através da reação da polimerase em cadeia (PCR) para os genes hblA, hblD e hblC (que codificam a hemolisina BL) e para os genes nheA, nheB e nheC (que codificam a enterotoxina não hemolítica - NHE). De um total de 70 isolados investigados, 14,3% foram positivos para os três genes da HBL e 12,8% foram positivos para os três genes da NHE. A produção de NHE também foi verificada através do... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Segurança alimentar; Hemolisina; Enterotoxina diarréica; PCR; ELISA. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782008000200033 |
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| Dubois, Rodolphe. |
| Bonamia ostreae, l'agent de la bonamiose, est un parasite intracellulaire ayant pour cible les hémocytes (cellules du système de défense) de l'huître plate ostrea edulis. Il induit chez son hôte des lésions branchiales, telles que des perforations ou des indentations pouvant aller jusqu'à la mort du mollusque. Le travail réalisé au cours de ce stage s'inscrivait dans une démarche de validation de méthode de PCR (Polymerase Chain Reaction) pour la détection de ce parasite. Les analyses ont porté sur un lot de trois cent onze huîtres plates ostrea edulis adultes (2-3 ans), issus de captage naturel et provenant de Quiberon (Bretagne). Pour chaque individu, une extraction d'ADN a été réalisée sur un échantillon de coeur et de branchies. Afin de déterminer si... |
| Tipo: Text |
Palavras-chave: Bonamia Ostreae; Agent pathogène; Bonamiose; Hémocytes; Huître plate; Ostrea edulis; PCR; Validation. |
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/00369/47989/48017.pdf |
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| Registros recuperados: 425 | |
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